This study aimed to evaluate the quality of frozen semen of Bali bull resulted
from sexing procedure on calf or offspring production with desired sex. The tested
sperm of Bali bull were collected from Bali bull raised at Regional Artificial
Insemination Center of Riau Province (BIBD Riau). The study was carried out in 2
stages. The first stage was X and Y chromosome separation by albumin method. The
extender used in the sexing procedure is trice citrate fructose and egg yolk. The second
stage was mainly testing the sexed sperm collected in 60 Bali cow in Langkat Village,
Bengkalis Regency. To determine the quality of post thawing frozen semen collected
from the sexing procedure, the study evaluated motility, viability, mortality, abnormality
and plasma membrane integrity of the spermatozoa. The pregnancy rate, calving rate,
and birth accuracy of inseminated sexed sperm to offspring’ sex were also evaluated.
The evaluation resulted in motility (66.3-75.3%), viability (70-78.5%), plasma
membrane integrity (60-65.8%), abnormality (6.05-8.05%), mortality (20.05-30.05%),
and pregnancy rate (83.33-90%). The calving rate on this study was 100% with the birth
accuracy of 81.8% for male offspring and 40% for female offspring. As conclusion, the
sexed sperm evaluated on this study have fairly good fertility