A. Riyuska Primary Author N.W.K. Karja Primary Author M.A. Setiadi Primary Author mixed material bibliography a Biology of Reproduction, Graduate School of Bogor Agricultural University 2018 en English

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The objective of this study was to determine the efficacy of leptin supplementation into maturation medium on nuclear maturation and fertilization rate of sheep oocytes. The maturation process was conducted using a tissue culture medium (TCM) 199 with four supplementation treatments of leptin namely 0 (control), 10, 50, and 100 ng/mL. Fertilization was conducted in the oocytes supplemented with 10 ng/mL and control using 5×106 mL-1 spermatozoa. At the end of maturation and fertilization processes, the oocytes were stained with 2% aceto orcein to determine nuclear maturation rate and pronuclear development. The results showed that the percentage of oocytes reaching metaphase II (MII) stage significantly increased in the oocytes supplemented with leptin at a dose of 10 ng/mL (P NONE pISSN2615787XeI UPT. Perpustakaan UNIMUDA Sorong Universitas Pendidikan Muhammadiyah (UNIMUDA) Sorong P01276S My Library 1861 2021-07-27 12:47:14 2021-07-27 12:47:14 machine generated