The research purpose was to carry out the effect of mannan-riched fraction
(MRF) and probiotic enhanced water as natural growth promoters (NGPs) on Growth
Performance, mortality, relative organ weight, blood biochemistry, and intestinal
microbial flora. A total of 3000 day old chicks (DOC) Arbor Acres broiler were
randomly allocated to 4 dietary treatments and 4 replications of 187 broilers per cage.
Four treatments used in research were as follows: i) CON, basal diet, ii) basal diet,
CON+ MRF (Actigen™) 80g/100kg/feed , iii) basal diet, CON+ 0.2% drinking water +
2 ml/L Combination feed additive (Acid-Pak 4-way®), and iv) ) basal diet, CON+ MRF
(Actigen™) 80g/100kg/feed+ drinking water 2 ml/L Combination feed additive (AcidPak 4-way®). The results showed that using mannan riched fraction (MRF) (feed) and
combination with probiotic-enhanced liquid acidifier (drinking water) presented
significant difference (P>0.05) on body weight gain at 1-28 days and intestinal
microbial. On the blood biochemistry, the effect of combination began to reduce the
amount of glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase
(GPT) at 21 days periods. To sum up, the addition of mannan-riched fraction and
combination with probiotic enhanced liquid acidifier doesn’t impacted on growth
Growth Performance, blood biochemistry, relative organ weight but give significant
effect on intestinal microbial and reduces mortality of broiler