Abstract. The exfoliative cytology of the vaginal was evaluated in Saanen goat during the
estrus cycle by vaginal smear method. Its a simple technique to determine of estrus cycle
stages and a useful tool to determine or identify the estrus phase. The aim of this study was to
determine the proportion of exfoliative vaginal cell during the stages of estrus cycle using
vaginal smear techniques in Saanen goat. Twenty Saanen goat with weigh 45-55 kg, 3-4 years
and had a period of estrus 19-22 days in dry period were observed. All goats were in natural
estrus cycle without synchronization. A vaginal smear and vaginal pH were collected from
swabbed vaginal epithelium. The result showed the proportion of vaginal cell was significantly
different in each phase of estrus cycle. The proportion of superficial cell was dominated in
estrus phase. Also, the highest pH values were significantly higher in estrus phase rather than
in the other phases. In conclusion, the dominant proportion of superficial cell and a high
vaginal pH value which occurred in follicular phase might be used as a marker to determine the
optimal time for goat insemination