Abstract. Citrus experienced physical damage in peel will produce essential oils that contain
polymethoxylated flavone. Polymethoxylated flavone is fluorescence substance; thus can be
detected by fluorescence imaging. This study aims to study the fluorescence spectra
characteristic and to determine the damage region in citrus peel based on fluorescence image.
Pulung citrus from Batu district, East Java, as a famous citrus production area in Indonesia, was
used in the experiment. It was observed that the image processing could detect the mechanical
damage region. Fluorescence imaging can be used to classify the citrus into two categories,
sound and defect citruses